Effect of Black Grape Seed(Vitis Vinifera) Extract Consumption with Moderate-Intensity Aerobic Training on the Expression of MicroRNAs in Type 1 Diabetic Cardiac Tissue Rats

Maryam Shirani Bidabadi; Jamshid  Banaei Borojeni; Saeed Keshavarz; Mohammad Karimi

doi:10.18502/ijdo.v13i4.8003

Maryam Shirani Bidabadi Sports Medicine Research Center, Najafabad Branch, Islamic Azad University, Najafabad, Iran.
Jamshid Banaei Borojeni Sports Medicine Research Center, Najafabad Branch, Islamic Azad University, Najafabad, Iran.
Saeed Keshavarz Sports Medicine Research Center, Najafabad Branch, Islamic Azad University, Najafabad, Iran.
Mohammad Karimi Professor Assistant of Exercise Physiology, Qom University of Technology, Qom, Iran.

DOI: https://doi.org/10.18502/ijdo.v13i4.8003

Keywords: Diabetes, Aerobic training, Grape Seed, miR-126, miR-29a

Abstract

Objective: This study aimed to evaluate the effect of consuming grape seed extract with moderate-intensity aerobic training on the expression of miR-126 and miR-29 in the cardiac tissue in type 1 diabetic male rats.

Materials and Methods: 40 rats with an initial weight range of 160-220 g were divided into five groups: Training + Extract, Training, Extract, Diabetic / Control, and Healthy / Control. Aerobic training program was moderate intensity and rats performed aerobic training for 60 minutes a day with the intensity 70 to 75% of maximum oxygen consumption (28 meters per minute). Grape seed extract was also administered by gavage at a dose of 40 mg/kg per day.

Results: Expression of both miRNAs in the three groups of training + extract, healthy training and control was significantly higher than the two groups of extract and diabetic control (P-value= 0.001). The difference between the three groups of training + extract, healthy training and control and also the difference between the two groups of extract and diabetic control were not significant (P-value> 0.05).

Conclusion: Aerobic training may be able to prevent cardiac disease caused by type 1 diabetes.