The Comparison of the Protective Effect of L-Carnitine, Chromium and Vitamin D with Metformin on Liver, Antioxidant Enzyme Activity, and Iron Metabolism in Diabetic Rats

Parisa Sheikh Samani; Kahin Shahanipour; Ali Noori Diziche; Mohammad Adibnejad

doi:10.18502/ijdo.v13i2.6476

Parisa Sheikh Samani Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Kahin Shahanipour Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Ali Noori Diziche Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Mohammad Adibnejad Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.

DOI: https://doi.org/10.18502/ijdo.v13i2.6476

Keywords: Diabetes mellitus, L-carnitine, Chromium, Vitamin D, Metformin

Abstract

Objective: Due to the lack of information about the positive or negative effects of L-carnitine, chromium, vitamin D, and the uptake of a safe dietary supplement to reduce the effects of diabetes, it seems essential to determine the effects of these drugs on diabetes.

Materials and Methods: Wistar rats were divided into 12 groups, control, diabetic, and intact, each of which was treated with metformin, L-carnitine, vitamin D, and chromium or a combination of L-carnitine, vitamin D, and chromium. The serum levels of liver function parameters, iron, TIBC, Catalase and GPX activities were measured. Data were statistically analyzed by one-way ANOVA using SPSS 18 software. Statistical significance among the groups was determined using LSD test, and P-value< 0.05 was considered significant in all cases.

Results: AST activity in diabetic groups and those receiving chromium and L-carnitine has significantly reduced (P-value= 0.009). A significant reduction in ALT and ALP activity in the diabetic groups receiving vitamin D and combined and non-diabetic groups receiving metformin were observed, in contrast to a significant increase in ALT activity in diabetic recipients of Lcarnitine (P-value= 0.009).

Conclusion: L-carnitine, chromium, and vitamin D supplements have synergistic effects and a combination of them has the best protective effect on factors that have been studied.