The Effect of Aerobic Training and L- Carnitine on BCL2 and Mitochondrial Enzymes of Rat's Kidney

Abstract

Objective: Diabetes causes free radical production, oxidative stress and alterations in mitochondrial enzymes and apoptosis. The purpose of this study was to investigate the effect of aerobic exercise and consumption of L-carnitine on BCL2 and some mitochondrial enzymes of the kidney in diabetic rats. Materials and Methods: In this experimental study, 45 male wistar rats (200-300 gr) were simple randomly divided into six groups: 1) placebo group, 2) healthy control group, 3) diabetic control group, 4) diabetic group receiving L- carnitine, 5) diabetic group of aerobic training and 6) diabetic group of aerobic training and recipient of L-carnitine. Rats with a serum glucose level higher than 300 mg/ dL were considered diabetic. L-carnitine recipients received 100 mg of L-carnitine daily orally. The dependent variables of the study were measured 24 hours after the last training program session by ELISA in kidney tissue. Data were analyzed by Shapiro- Wilk and two way ANOVA and Tukey post hoc test at P -value< 0.05. Results: The results showed that the combined effect of aerobic training and supplementation of L-carnitine on Bcl2 factor of kidney tissue of rats with diabetes has a significant effect ( P -value: 0.019). But aerobic exercise ( P -value: 0.969) and supplementation of Lcarnitine ( P -value: 0.584) were not significant. Conclusion: The results of this study showed that the combined effect of aerobic exercise and supplementation of L-carnitine on CPT2 and Malonyl COa and Bcl2 have a significant effect on the kidney of diabetic rats. Also, exercise alone and consumption of Lcarnitine alone do not have a significant effect.