BCL2 Expression in the Brains of Diabetic Rats Treated with Ginger

Mohammad Mohsen  Taghavi; Seyed Hassan  Eftekhar-Vaghefi; Zahra  Taghipour; Ahmad  Shabanizadeh; Abdolreza  Babaee; Ilia  Najmadini; Akram  Molahosseini

doi:10.18502/ijdo.v17i3.19264

Mohammad Mohsen Taghavi Department of Anatomy, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Seyed Hassan Eftekhar-Vaghefi Department of Anatomy, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Zahra Taghipour Department of Anatomy, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Ahmad Shabanizadeh Department of Anatomical Sciences, Medical School, Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Abdolreza Babaee Department of Anatomy, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Ilia Najmadini Student in medical science, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Akram Molahosseini Department of Anatomy, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

DOI: https://doi.org/10.18502/ijdo.v17i3.19264

Keywords: Apoptosis, Glycaemia, Ginger, Hippocampus

Abstract

Objective: Diabetes is one of the most common high-risk diseases which causes many side effects in most body systems such as the nervous system. The medicinal plants, has been suggested to reduce the side effects of this diseases. In this experimental study, we examined the effect of ginger on BCL2 gene expression in the hippocampus of diabetic rats.

Materials and Methods: In this experimental study, we used 60 Wistar rats (200gr). We separated the rats into one healthy group and four groups of diabetics which after seven days of diabetes induction via Streptozotocin (60 mg/kg), received different mixture of insulin and ginger for 6 weeks. 24 hours after the last injection, brains of rats were removed and the hippocampus region was studied histologically and the BCL2 gene expression examined by Real-time PCR.

Results: According to the results, the blood glucose level in the ginger treated group (183.17) decreased significantly compared to the diabetic control group (232.5) (P˂ 0.01). In the expression of BCL2 gene, there was not any significant difference among the groups. In the histological examination of the hippocampus CA1 region, the ginger-insulin treated group had less pyknotic neurons than the diabetic control group.

Conclusion: According to results, the ginger, in addition to glycaemia reduction and despite any significant change in BCL2 gene expression, can cause the safe neurons with the bright nucleus and clear nucleolus remain in the treated group, which can indicate that maybe ginger could inhibit the creation of apoptosis