Effects of Lipoic Acid and High-Intensity Interval Training (HIIT) on Pancreatic VEGFR-3 Levels in Diabetic Rat Model

Seyed Ramin Hashemian Esfahani; Minoo Dadban Shahamat; Asra Askari; Abbas Nezhadebrahimi

doi:10.18502/ijdo.v16i2.15709

Seyed Ramin Hashemian Esfahani Department of Physical Education, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran.
Minoo Dadban Shahamat Department of Physical Education, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran.
Asra Askari Department of Physical Education, Gorgan Branch, Islamic Azad University, Gorgan, Iran.
Abbas Nezhadebrahimi Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran.

DOI: https://doi.org/10.18502/ijdo.v16i2.15709

Keywords: ALA, HIIT, Lymph angiogenesis, Diabetic model rats

Abstract

Objective: The purpose of this experimental research is to investigate the effects of High-Intensity Interval Training (HIIT) and Lipoic Acid (ALA) supplementation on VEGFR-3 of pancreatic in diabetic Wistar rats model.

Materials and Methods: 20 male Wistar rats weighing 159 ± 3 gr and aged3 weeks, were randomly assigned into 4 groups:1) diabetes/sham, 2)diabetes/ ALA, 3)diabetes/exercise/sham, and 4) diabetes /exercise/ ALA. Diabetes was induced with streptozotocin (65 mg/kg dose) and Nicotinamide (120 mg/kg dose). After two weeks of familiarization with interval training, the rats started their main training, included 10 repetitions of 4 minutes of running on the treadmill with an intensity of 85-90% VO2max and 2 minutes of active rest between repetitions (5-10 m/min) for 5 sessions per week for 6 weeks. ALA supplement was taken at a dose of 20 mg/kg/day for 6 weeks. One-way ANOVA test used and Tukey's post hoc test at for analysis (P≤ 0.05).

Results: HIIT has a significant effect on blood glucose (P= 0.004) and insulin (P= 0.001) and VEGFR3 (P= 0.001) of pancreatic tissue of diabetic rats.

Conclusion: Lymphatic vessels play an important role in the pancreas and treat diabetes.The results of this research showed that HIIT and ALA increased lymphangiogenesis in the diabetic rat model.