Evaluation of rs1205 Polymorphism in C-Reactive Protein Encodind Gene and its Association with Serum Low-Density Lipoprotein in Iranian Population

Elham  Imanian; Vida  Hojati; Farid  Ebnerasuly

doi:10.18502/ijdl.v25i5.20343

Elham Imanian Department of Biology, Da.C., Islamic Azad University, Damghan, Iran
Vida Hojati Department of Biology, Da.C., Islamic Azad University, Damghan, Iran
Farid Ebnerasuly Department of Biology, SR.C., Islamic Azad University, Tehran, Iran

DOI: https://doi.org/10.18502/ijdl.v25i5.20343

Keywords: C-Reactive Protein Encoding Gene, CRP Gene, rs1205 Polymorphism, Low-Density Lipoprotein, Blood Fat

Abstract

Background: Blood fat is a global problem and one of the major threats to society's health. Hyperlipidemia is considered a multigenic disease, most of the genes related to it remain unknown. rs1205 is one of the polymorphisms of the C- Reactive Protein (CRP) gene that causes the change of the nucleotide C to T and is one of the risk factors for increasing low-density lipoprotein (LDL) level. This study aimed to investigate the prevalence of rs1205 polymorphism in CRP gene and its relationship with LDL level in Iranian population.

Methods: The total number of samples was 137, including 79 controls and 58 patients (LDL above 130 mg/dL). Then the samples were analyzed using the amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) method for the presence or absence of rs1205 polymorphism in the CRP gene. The PCR product was transferred on agarose gel. After observing the bands and checking their correctness, different alleles were examined.

Results: 56.2% of the samples had a body mass index (BMI) lower than 25 and 43.8% had a BMI higher than 25. The frequency percentage of genotypes showed that CT genotype is equal to 47.4%, CC genotype is equal to 36.5% and TT genotype is equal to 16.1%. 57.7% of the samples had LDL below 130 and 42.3% had LDL above 130.

Conclusion: A significant relationship wasn't observed between the rs1205 polymorphism and the serum LDL level of the studied population. Also, the difference in age and gender of the samples had no effect on this relationship. It is possible that the lack of difference in ethnicity is one of the possible reasons for the non-significance of the results of this study.