The Effect of Aerobic Exercise on Ferritin Levels and Antioxidant System in Sarcopenia Model Mice

Afshin Khaman  hatami; Kamal  Azizbeigi; Zaher  Etemad; Parvin  Farzanagi

doi:10.18502/ijdl.v25i5.20341

Afshin Khaman hatami Department of Physical Education, Sa.C., Islamic Azad University, Sanandaj, Iran
Kamal Azizbeigi Department of Physical Education, Sa.C., Islamic Azad University, Sanandaj, Iran
Zaher Etemad Department of Physical Education, Sa.C., Islamic Azad University, Sanandaj, Iran
Parvin Farzanagi Department of Physical Education, S.C., Islamic Azad University, Sari, Iran

DOI: https://doi.org/10.18502/ijdl.v25i5.20341

Keywords: Aerobic training, Ferroptosis, Oxidative stress, Sarcopenia

Abstract

Background: Aging is associated with Sarcopenia as well as oxidative stress (OS) caused by Ferroptosis. The aim of the present study was to investigate the effect of aerobic exercise training (AT) on OS caused by ferroptosis in sarcopenia model mice.

Methods: Twenty-one C57BL/6 mice (16-24 wks. age) sarcopenia model with an average weight (25-35 g), and 21 healthy mice (6-7 weeks age) were randomly assigned to the following (1) healthy-young control (HYC; n= 7), (2) aerobic training-young (ATY; n= 7), (3) healthy-old control (HOC; n= 7), (4) old control-sarcopenia model (OCS; n= 7), (5) aerobic training-healthy-old (ATHO; n= 7), (6) healthy-old sarcopenia model (HOS= 7). The intervention group underwent AT for eight weeks, five sessions per week at an intensity of 60-80% of aerobic capacity (VO2max). The expression of the glutathione peroxidase (GPX-4) and superoxide dismutase (SOD) genes was measured using Real Time PCR. The Malondialdehyde (MDA) and Fe²⁺concentration in the gastrocnemius muscle was also measured.

Results: It was observed that the enzymes gene expression of SOD and GPX-4 in the (OSC) was significantly decreased compared to the (HOC) (P= 0.001, P= 0.002, respectively), and the MDA and Fe²⁺ was significantly increased (P= 0.001; P= 0.002, respectively). Also, the of SOD and GPX-4 gene expression of enzymes in the ATY, ATHO was significantly increased compared to the HYC, and HOS (P= 0.001; P= 0.002, respectively), and the concentration of MDA and Fe²⁺ was significantly decreased (P= 0.001; P= 0.002, respectively).

Conclusion: Finally, it can be said that aerobic training reduces oxidative stress caused by ferroptosis in Sarcopenia by increasing the enzymes GPX and SOD gene expression and reducing the concentration of MDA and Fe²