In-Vitro Studying Toxicity of Herbal Extracts Mixture in Normal Environment and Protective Effect of Mixtures in High-Glucose Environment

Alireza  Moumivand; Bahareh  Tavakoli-far; Gelareh  Vahabzadeh; Saeideh  Momtaz; Maliheh  Farid; Hosein  Rafiemanesh; Mahdi  Goudarzvand

doi:10.18502/ijdl.v25i3.20234

Alireza Moumivand Alborz University of Medical Sciences, Karaj, Iran
Bahareh Tavakoli-far Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran
Gelareh Vahabzadeh Department of Pharmacology, Iran University of Medical Sciences, Tehran, Iran
Saeideh Momtaz Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
Maliheh Farid Non-communicable Diseases Research Centre, Alborz University of Medical Sciences, Karaj, Iran
Hosein Rafiemanesh Non-communicable Diseases Research Centre, Alborz University of Medical Sciences, Karaj, Iran
Mahdi Goudarzvand Department of Physiology- Pharmacology- Medical Physics, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran

DOI: https://doi.org/10.18502/ijdl.v25i3.20234

Keywords: PC12 Neurons, Herbal Extracts, Diabetes, Cell Culture

Abstract

Background: Diabetes is one of the most common disorders of the endocrine glands, the main characteristic of which is an increase in the concentration of glucose in the serum of patients. Herbal medicines are widely accepted by people due to less side effects. The purpose of this study is to investigate the protective effect of the silybum marianum, melissa officinalis, vaccinium arctostaphylos, trigonella foenum, urtica dioica and citrullus colocynthis extracts mixtures in the PC12 cell model in a high glucose environment.

Methods: After 24, 48, and 72 hours of adding different concentrations of plant extracts (silybum marianum, melissa officinalis, vaccinium arctostaphylos, trigonella foenum, urtica dioica and citrullus colocynthis) in the normal culture medium of PC12 cells and the medium with high glucose (25 mg/ml, 13/5), cell viability was measured by MTT method.

Results: The results showed that the viability of PC12 cells did not change in 24, 48 and 72 hours after treatment with a mixture of plant extracts. The survival rate of cells in the high dose glucose group was significantly reduced compared to the control group, and the mixture of plant extracts in high doses significantly reduced cell death in these conditions.

Conclusion: Based on the available findings, glucose with a concentration of 25, 13.5 mg/ml caused the death of PC12 cells and the mixture of plant extracts was able to reduce the cell death caused by high glucose after exposure with nerve cells.