The Effect of Magnesium Supplementation and Training on The Content of CREB2 and C/EBP Homologous Proteins in the Left Ventricle of the Heart of Type 2 Diabetic Rats

Fatemeh  Solhdous; Mohammad Sherafati  Moghadam; Mitra  Tajari

doi:10.18502/ijdl.v25i1.20213

Fatemeh Solhdous Department of Sport Sciences, Apadana Institute of Higher Education, Shiraz, Iran
Mohammad Sherafati Moghadam Department of Sport Sciences, Apadana Institute of Higher Education, Shiraz, Iran
Mitra Tajari Department of Physical Education and Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran

DOI: https://doi.org/10.18502/ijdl.v25i1.20213

Keywords: Magnesium Supplementation, Exercise Training, Type 2 Diabetes, Left Heart Ventricle

Abstract

Background: Diabetes can disrupt the balance of cell death through different cell pathways, and exercise or consumption supplements can be effective in maintaining the balance of cell death types; Therefore, the purpose of this research is the effect of magnesium supplementation and exercise training on the content of CREB2 and C/EBP homologous protein (CHOP) in the left ventricle of the heart of type 2 diabetic rats.

Methods: In this experimental study, 24 2-month-old male Sprague-Dawley rats with an average weight of 280±20 grams were selected. Type 2 diabetes was induced by injecting nicotinamide and streptozotocin solutions. The rats were randomly divided into four groups, 1) control, 2) supplement, 3) training and 4) training+supplement; Resistance training consisted of 8 weeks and 3 weekly sessions of climbing a ladder. Magnesium supplement was given to rats once a day. To analyze the data, one-way ANOVA and Tukey's post hoc tests were used in SPSS version 29.

Results: Eight weeks of magnesium supplementation and resistance training led to a significant change in the content of CREB2 and CHOP proteins between groups in the left ventricle of the heart (P= 0.001). A significant decrease was observed in the groups of resistance training + magnesium supplement and magnesium supplement compared to the control group (P≥ 0.05); But the resistance training group had increased compared to the control group (P≥ 0.05).

Conclusion: The increase and decrease of CREB2 and CHOP proteins in the left ventricle of the heart can lead to improvement and physiological adaptation, like a bilateral mechanism