Alteration in the expression of PELP1 and c-Src genes in tumor tissue of colorectal cancer patients

Nina  Lotfi; Fatemeh  Babajani; Seyed Askar  Roghani; Atefeh  Kakavand; Mohammad Taghi  Goodarzi; Soheila  Asadi

doi:10.18502/abi.v3i4.21851

Nina Lotfi Department of Biochemistry, Sha.C., Islamic Azad University, Shahrood, Iran
Fatemeh Babajani Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran
Seyed Askar Roghani Clinical Research Development Center, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran
Atefeh Kakavand Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran
Mohammad Taghi Goodarzi Department of Biochemistry, Sha.C., Islamic Azad University, Shahrood, Iran
Soheila Asadi Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran

DOI: https://doi.org/10.18502/abi.v3i4.21851

Keywords: c-Src, PELP1, Colorectal cancer, gene expression

Abstract

Objectives: The genetic and environmental factors have crucial role in colorectal cancer (CRC) pathogenesis. Due to important role of proline , glutamic acid, and leucine-rich protein 1 (PELP1) and c-Src genes in different types of malignancy, this study aimed to investigate the expression levels of the PELP1 and c-Src genes in tumor versus matched non-cancerous margin tissues of patients with CRC, and further evaluate their capacity as potential diagnostic biomarkers.

Methods: The gene expression of PELP1 and c-Src in 31 tumor tissues and 31 non- cancerous margin tissues of CRC subjects was analyzed by the Real-Time PCR. Moreover, Receiver Operating Characteristic (ROC) curve analysis was utilized for the determination of these genes’ RNA levels as potential biomarkers.

Results: Our findings indicated the increased PELP1 (P=0.016) and c-Src (P=0.006) gene expression in tumor tissues, compared to non-cancerous margin tissues in CRC. The result of ROC analysis showed that the area under the curve (AUC) for PELP1 and c-Src were 0.673 (Cut off:7.74, sensitivity:0.714, specificity:0.615) and 0.731 (Cut off:16.04, sensitivity: 0.857, specificity: 0.653), respectively.

Conclusion: The higher expression of c-Src and PELP1 genes in tumor tissues compared to non-cancerous margin tissues indicated that these genes are critical components of the signaling pathways involved in CRC pathogenesis. Furthermore, the findings revealed that studied genes can have a potential for diagnosis purposes in CRC.