Glutathione Peroxidase 1 Pro198Leu Polymorphism and Susceptibility to Rheumatoid Arthritis: Evidence from an Iranian Case-Control Study

Arshia  Abbaszadeh; Mohammad Reza  Asgharzadeh; Zafar  Gholinejad

doi:10.18502/abi.v3i4.21846

Arshia Abbaszadeh Department of Biology, Ur.C., Islamic Azad University, Urmia, Iran
Mohammad Reza Asgharzadeh Department of Biology, Ur.C., Islamic Azad University, Urmia, Iran
Zafar Gholinejad Biotechnology Research Center, Ur.C., Islamic Azad University, Urmia, Iran

DOI: https://doi.org/10.18502/abi.v3i4.21846

Keywords: Rheumatoid Arthritis, GPx-1, Pro198Leu, Single Nucleotide Polymorphism

Abstract

Objectives: Oxidative stress plays a key role in the pathogenesis of rheumatoid arthritis (RA). Glutathione peroxidase (GPx) is a peroxidase enzyme that defends mammalian cells against oxidative stress. The Pro198Leu polymorphism of the GPx-1 gene, has been reported to influence its activity. This study aimed to investigate the association of this polymorphism with RA risk in an Iranian population.

Methods: In this case-control study, 45 RA patients and 45 healthy controls were enrolled. Genomic DNA was extracted from blood samples, and genotyping for the Pro198Leu polymorphism was performed using the PCR-RFLP technique. Statistical analysis was conducted using SPSS software, employing chi-square tests and ANOVA.

Results: The mean age of all participants was 55.69 years. Genotype distribution was as follows: CC (50.0%), CT (44.4%), and TT (5.6%). Chi-square analysis revealed no significant difference in genotype frequencies between patients and controls (p = 0.779). Furthermore, no significant association was found between genotypes and age or gender. All groups were in Hardy-Weinberg equilibrium.

Conclusion: The findings indicate that the GPx-1 Pro198Leu polymorphism is not significantly associated with the risk of developing RA in the studied population.