Evaluation of Leucomethylene Blue as a Protective Agent Against Acetaminophen-Induced Acute Lung Injury

Majid  efati; Sahar  Ghoflchi; Khatereh  Kharazmi; Soodeh  Alidadi; Daryoush  Hamidi-alamdari; Hossein  Hosseini

doi:10.18502/abi.v3i2.19488

Majid efati Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Sahar Ghoflchi Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Khatereh Kharazmi Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Soodeh Alidadi Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Daryoush Hamidi-alamdari Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Hossein Hosseini Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

DOI: https://doi.org/10.18502/abi.v3i2.19488

Keywords: LeucoMethylene Blue, lung injury, Acetaminophen, Inflammation, Oxidative stress

Abstract

Objectives: Acetaminophen overdose may lead to acute pulmonary complications, such as acute lung injury, due to its harmful effects on cellular systems caused by oxidative stress. Leucomethylene blue (LMB) may have beneficial effects by improving hemodynamic stability and reducing oxidative damage through its nitric oxide synthase inhibitory and antioxidant activities. This study aimed to evaluate the effect of LMB on acetaminophen-induced pulmonary injury in rats.

Methods: Lung samples were collected from 30 male Wistar rats, which were randomly divided into five groups and frozen for later analysis. The groups included control, acetaminophen, N-acetylcysteine (NAC)-treated, LMB-treated, and NAC+LMB combination-treated. We evaluated total antioxidant capacity (TAC), glutathione reductase (GR), TNF-α and IL-6 levels, histopathology, and relevant tissue remodeling changes.

Results: Our results demonstrated that the administration of LMB significantly diminished the oxidative and inflammatory damage caused by APAP toxicity in the lungs. LMB restored TAC and GR activity, which were significantly reduced by APAP toxicity. Additionally, LMB restricted the overproduction of pro-inflammatory cytokines released from lung tissue. Moreover, LMB substantially counteracted the pulmonary lesions caused by APAP, including edema, hemorrhage, and infiltration of inflammatory cells, as confirmed by histopathological analysis.

Conclusion: The results of this study show that LMB can effectively reduce lung damage caused by acetaminophen poisoning