Assessment of resistance and aerobic training with/ without Blood Flow Restriction and detraining period and their association with miR143/145 (rs4705342 and rs4705343) and IGF2BP2 (rs4402960 and rs1470579) gene polymorphisms in men with type 2 diabetes

Hassan  Moudi; Foad  Feizolahi; Alireza  Rahimi; Ramin  Saravani

doi:10.18502/abi.v3i2.19484

Hassan Moudi Department of Exercise Physiology, Ka. C., Islamic Azad University, Karaj, Iran
Foad Feizolahi Department of Exercise Physiology, Ka. C., Islamic Azad University, Karaj, Iran
Alireza Rahimi Department of Exercise Physiology, Ka. C., Islamic Azad University, Karaj, Iran
Ramin Saravani Cellular and Molecular Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran

DOI: https://doi.org/10.18502/abi.v3i2.19484

Keywords: Type 2 diabetes (T2D), resistance training (RT), aerobic training (AT), Blood Flow Restriction (BFR), miR143/145, IGF2BP2, polymorphisms.

Abstract

Objectives: Type 2 diabetes (T2D) is a prevalent metabolic disorder characterized by insulin resistance and impaired glucose metabolism, often leading to severe complications. Emerging evidence suggests that exercise, particularly resistance training and aerobic activities, can significantly improve glycemic control and overall health in individuals with T2D. This study aimed to assess resistance and aerobic training, both with and without blood flow restriction (BFR), and genetic polymorphisms located in the miR-143/145 and IGF2BP2 gene clusters in men with T2D.

Methods: A total of 30 men with T2D were randomly assigned to four groups: resistance training with BFR (RT-BFR), resistance training without BFR (RT), aerobic training with BFR (AT-BFR), aerobic training without BFR (AT), and two control groups. Training sessions were conducted three times per week for 12 weeks, followed by a 6-week detraining period. Genotyping was performed for polymorphisms within the miR-143/145 and IGF2BP2 gene clusters using ARMS-PCR.

Results: The results of our study showed that in the AT group, the dominant genotype was TT rs4705342 TT rs4705343 GG rs4402960 AA rs1470579. In the RT group and the Control AT group, the dominant genotype was TT rs4705342 TT rs4705343 GG rs4402960 CC rs1470579. In other groups (including AT-BFR, RT-BFR, and Control RT groups), the dominant genotype was TT rs4705342 TT rs4705343 GG rs4402960 AC rs1470579.
The results were significant for AT vs. Control AT at the rs4402960 position in the recessive model. Therefore, the risk decreased by 0.74 for TT vs. GT+GG (p = 0.025). Moreover, the RT group vs. Control RT group at the rs1470579 position in the same model yielded significant results, leading to a 14-fold increase in risk for CC vs. AC+AA (p < 0.001).

Conclusion: The findings from this research contribute valuable evidence to the ongoing discourse surrounding exercise, genetics, and diabetes management.