Whole-genome sequencing of Salmonella phage vB_SenS_ TUMS_E15 for bio-control in the food chain

Narges Torkashvand; Haniyeh Kamyab; Ahmad Reza Shahverdi; Mohammad Reza Khoshayand; Mohammad Amir  Karimi Tarshizi; Zargham  Sepehrizadeh

doi:10.18502/abi.v2i1.16245

Narges Torkashvand Department of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Haniyeh Kamyab Department of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Ahmad Reza Shahverdi Department of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Mohammad Reza Khoshayand Department of Food and Drug Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad Amir Karimi Tarshizi Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
Zargham Sepehrizadeh Department of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran.

DOI: https://doi.org/10.18502/abi.v2i1.16245

Keywords: Bacteriophage, Salmonella enteritidis, Jersyvirus, Guernseyvirinae, Biocontrol

Abstract

The genome analysis of bacteriophages is crucial for their successful application in clinical and biocontrol settings. In this study, we isolated a new lytic phage, vB_SenS_TUMS-E15, from hospital sewage that was effective against Salmonella enteritidis, and analyzed its genomic features. The complete genome analysis revealed that E15 had circularly permuted double-stranded DNA of 43,048 base pairs (bp), with a G+C content of 49.7%. Sixty coding sequences (CDSs) were predicted in the genome, with 44 CDSs encoding known proteins in different modules, including packaging, structure, replication, metabolism, and lysis modules. No tRNA genes were found in the genome. Eight transcriptional promoter sequences and 37 rho-independent terminators were detected in the E15 genome. Phylogenetic analysis based on whole-genome sequences suggested that phage E15 should be classified as a member of the Jersyvirus genus in the subfamily Guernseyvirinae. Furthermore, no antibiotic-resistance genes, toxins, virulence factors, or lysogen-forming genes were observed in the genome. This suggests that E15 is a lytic phage, making it a promising candidate for clinical and biocontrol purposes.